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. 2015 Jul 20;6(28):26052–26064. doi: 10.18632/oncotarget.4482

Figure 4. rhLIF's regulation of the anti-apoptotic protein Mcl-1, and caspases 3/7 activity via Mcl-1, in CCA cells.

Figure 4

By WB, rhLIF induced a significant up-regulation of Mcl-1 (anti-apoptotic) (A, B. black columns), in both HuCCT-1 A. and TFK-1 B. cells compared with untreated cells; this effect was abrogated by the specific PI3K inhibitor, LY294002 (A, B gray columns). Representative blots are shown below each respective graph. Interestingly, the protective effect of rhLIF pre-treatment from GEM+CDDP (Mix) cytotoxicity (C, D. black columns) was abolished by UMI-77, a selective, small molecule inhibitor of Mcl-1 (C, D dark gray columns) (*p < 0.05 vs. untreated; ^p < 0.01 vs. untreated; •p < 0.01 vs. LIF 10 without LY294002; °p < 0.05 vs. LIF 100; §p < 0.05 vs Mix treatment; §§p < 0.01 vs Mix treatment; n = minimum of 3).