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. 2015 Jul 20;6(28):26161–26176. doi: 10.18632/oncotarget.4544

Figure 2. NDRG2 inhibits the expression of glucose and glutamine transporters, glycolytic and glutaminolytic enzymes in colorectal cancer cells.

Figure 2

A. NDRG2, GLUT1, HK2, PKM2, and LDHA mRNA and protein levels in Caco-2, HT-29 and HCT116 cells infected with lentivirus containing NDRG2 or mCherry, and β-actin served as an internal control to ensure equal loading. B. NDRG2, ASCT2 and GLS1 mRNA and protein levels in HCT116 and LS 174T cells infected with lentivirus containing NDRG2 or mCherry, and β-actin served as an internal control to ensure equal loading. C. Glucose transport activity measured using the fluorescent glucose analog 2-NBDG and D. glycolytic enzymes HK, PYK and LDH activity in HT-29 cells infected with lentivirus containing NDRG2 or mCherry. E. NDRG2, GLUT1, HK2, PKM2, and LDHA mRNA and protein levels in Caco-2, HT-29 and HCT116 cells infected with lentivirus containing NDRG2 shRNA or control shRNA, and β-actin served as an internal control to ensure equal loading. F. NDRG2, ASCT2 and GLS1 mRNA and protein levels in HCT116 and LS 174T cells infected with lentivirus containing NDRG2 shRNA or control shRNA, and β-actin served as an internal control to ensure equal loading. G. Glucose transport activity measured using the fluorescent glucose analog 2-NBDG and H. glycolytic enzymes HK, PYK and LDH activity in HT-29 cells infected with lentivirus containing NDRG2 shRNA or control shRNA. All *P < 0.05; **P < 0.01.