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. 2015 Jul 3;6(28):26308–26321. doi: 10.18632/oncotarget.4763

Figure 5. In vitro ubiquitination of P-gp (time course).

Figure 5

Purified in vitro translated FLAG-tagged P-gp along with E2 (UbcH5c), E3 ligase FBXO21, Cul1, Skp1, Roc1 purified from Sf-9 insect cells were incubated in vitro. Reactions were started by adding ATP and stopped by adding sample buffer at different time points. Samples were analyzed by immunoblotting and anti-FLAG antibody.