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Following genes were analysed; the pluripotent markers POU5F1 (a) and NANOG (b); mesendoderm/early DE marker BRACHUYRY (c); the DE markers GOOSECIOD (d), SOX17 (e), FOXA2 (f); the WNT downstream target AXIN2 (g); and the WNT inhibitor DKK1. (n = 3, mean ± S.E.M.). Abbreviations are explained in legend Fig 3.
