Table 2. Acyl chain composition of WEs synthesized by cultures expressing mouse AWAT2 and AWAT2-derived domain swap variants in comparison with the acyl CoA-pool composition upon feeding of 18:1-OH.
| acyl chain | AWAT2 | V1 | V2 | V5 | acyl-CoA pool |
|---|---|---|---|---|---|
| 16:1 | 0.54 ± 0.03 b | 0.50 ± 0.05 b | 0.36 ± 0.04 a | 0.39 ± 0.03 a , b | 0.33 ± 0.06 a |
| 16:0 | 0.16 ± 0.05 b | 0.16 ± 0.03 b | 0.13 ± 0.02 b | 0.12 ± 0.02 b | 0.09 ± 0.01 a |
| 18:1 | 0.24 ± 0.04 | 0.28 ± 0.02 b | 0.27 ± 0.05 | 0.27 ± 0.05 | 0.23 ± 0.03 |
| 18:0 | 0.05 ± 0.01 b | 0.05 ± 0.01 b | 0.12 ± 0.02 a , b | 0.09 ± 0.01 a , b | 0.07 ± 0.01 a |
| >C18 | 0.01 ± 0.01 b | 0.01 ± 0.01 b | 0.13 ± 0.01 b | 0.12 ± 0.03 a , b | 0.28 ± 0.07 a |
a Values are significantly different from respective AWAT2 values as deduced from a student’s t-test (p ≤ 0.05).
b Values are significantly different from respective acyl-CoA pool values as deduced from a student’s t-test (p ≤ 0.05).
Data represent mean and standard deviation of samples derived from at least three independent biological replicates.