Table 3. Acyl chain composition of WEs synthesized by cultures expressing mouse AWAT2 and AWAT2-derived single amino acid exchange variants in comparison with the acyl CoA-pool composition upon feeding of 16:0-OH.
| acyl chain | AWAT2 | A25F | A25F N36R | N36R | acyl-CoA pool |
|---|---|---|---|---|---|
| 16:1 | 0.57 ± 0.03 b | 0.58 ± 0.01 b | 0.28 ± 0.01 a , b | 0.27 ± 0.01 a , b | 0.32 ± 0.06 a |
| 16:0 | 0.14 ± 0.01 b | 0.09 ± 0.00 a , b | 0.09 ± 0.00 a , b | 0.09 ± 0.01 a , b | 0.08 ± 0.01 a |
| 18:1 | 0.24 ± 0.02 b | 0.27 ± 0.01 a , b | 0.25 ± 0.01 b | 0.22 ± 0.03 b | 0.32 ± 0.04 a |
| 18:0 | 0.04 ± 0.01 | 0.04 ± 0.00 | 0.11 ± 0.00 a , b | 0.12 ± 0.01 a , b | 0.04 ± 0.01 |
| >C18 | 0.00 ± 0.01 b | 0.02 ± 0.01 a , b | 0.27 ± 0.00 a | 0.30 ± 0.02 a , b | 0.24 ± 0.05 a |
a Values are significantly different from respective AWAT2 values as deduced from a student’s t-test (p ≤ 0.05).
b Values are significantly different from respective acyl-CoA pool values as deduced from a student’s t-test (p ≤ 0.05).
Data represent mean and standard deviation of samples derived from at least three independent biological replicates.