Table 4. Acyl chain composition of WEs synthesized by cultures expressing mouse AWAT2 and AWAT2-derived single amino acid exchange variants in comparison with the acyl CoA-pool composition upon feeding of 18:1-OH.

acyl chain	AWAT2	A25F	A25F N36R	N36R	acyl-CoA pool
16:1	0.54 ± 0.03 b	0.48 ± 0.02 a , b	0.38 ± 0.02 a	0.32 ± 0.05 a	0.33 ± 0.06 a
16:0	0.16 ± 0.05 b	0.15 ± 0.02 b	0.11 ± 0.01 a b	0.14 ± 0.02 b	0.09 ± 0.01 a
18:1	0.24 ± 0.04	0.27 ± 0.03 b	0.28 ± 0.02 b	0.24 ± 0.05	0.23 ± 0.03
18:0	0.05 ± 0.01 b	0.07 ± 0.01	0.12 ± 0.01 a , b	0.12 ± 0.02 a , b	0.07 ± 0.01 a
>C18	0.01 ± 0.01 b	0.03 ± 0.02 a , b	0.12 ± 0.04 a , b	0.14 ± 0.06 a b	0.28 ± 0.07 a

^a Values are significantly different from respective AWAT2 values as deduced from a student’s t-test (p ≤ 0.05).

^b Values are significantly different from respective acyl-CoA pool values as deduced from a student’s t-test (p ≤ 0.05).

Data represent mean and standard deviation of samples derived from at least three independent biological replicates.