Figure 3. Tumorigenic potential of low passage mucoepidermoid carcinoma cells sorted for ALDH/CD44.

A., B. Graphs depicting tumor volume of A. UM-HMC-3A or B. UM-HMC-3B xenograft cells FACS-sorted for ALDH/CD44. Scaffolds were seeded with either 400 ALDH^highCD44^high or 4,000 ALDH^lowCD44^low cells and transplanted into the subcutaneous space of SCID mice. Existing tumors were retrieved, re-sorted and 400 ALDH^highCD44^high or 4,000 ALDH^lowCD44^low cells seeded into new scaffolds, and serially passaged in vivo. C. Table depicting the number of tumors grown in the ALDH^highCD44^high versus ALDH^lowCD44^low populations for each passage performed. D. H&E staining of tumors generated with FACS-sorted ALDH^highCD44^high and ALDH^lowCD44^low cells. Images were taken at 100X. E. UM-HMC-3A and UM-HMC-3B cells were sorted for ALDH^highCD44^high or combined ALDH^highCD44^low, ALDH^lowCD44^high, and ALDH^lowCD44^low (non-CSC population). NP-40 lysis buffer was used to prepare whole cell lysates that were resolved using PAGE. Membranes were probed using antibodies a 1:1000 dilution against human mTor, p-mTor, Akt, p-Akt, S6K, p-S6K, p-EGFR; 1:2000 dilution of EGFR, and beta-actin.