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. 2015 Sep 22;6(29):26651–26662. doi: 10.18632/oncotarget.5791

Figure 6. Normal colon mucosa cells from colon cancer patients are not sensitive to treatment with an LXR agonist.

Figure 6

A. Western blot analysis of RXRα and t-RXRα protein expression in Tumoral (Tum) or healthy peripheral Non-tumoral (N) tissues from colon cancer patients. β-Actin was used as a loading control. Numbers indicate molecular masses in kilodaltons. B. Mean of the quantification of the RXRα/β-actin (black) and the t-RXRα/ β-actin (grey) ratios in Tumoral or Non-Tumoral tissues from ten colon cancer patients. C. Immunohistochemical staining of LXRβ in non-tumoral colonic mucosa (upper panel) and tumoral tissue (lower panel) from human colon cancer patients. One representative patient out of ten. D. Mean relative quantification of the LXRβ staining in the nucleus in tumoral (red) or non-tumoral cells (blue) in ten different patients ± s.d.. E. FLICA-1 positive cells in tumoral (Tum- red circles) or Non-tumoral (N - blue diamonds) tissues of colon cancer patients treated ex-vivo with 20μM T0901317 or the vehicle (control) for one hour. Statistics compare tumors with Non-tumor samples: **p < 0.005, n.s., not significant (B. and D.) or T0901317-treated samples with untreated samples (control): *p < 0.05, n.s., not significant E., using two tailed t test.