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. 2015 Sep 10;6(29):26690–26701. doi: 10.18632/oncotarget.5614

Figure 1. Changes in levels and distribution of mitochondrial fission proteins in the ageing retina of C57BL/6 mice.

Figure 1

A. Retinal sections of both young and old mice were immunostained with a mitochondrial fission antibody, Fis1 (red) and the nuclei counterstained with 4′, 6-diamino-2-phenylindole (DAPI) (Blue). Expression of Fis1 is stronger in the ganglion cell layer of the young retina compared to the old, while the old retina express more Fis1 in the photoreceptor layer than the young. B. Graph showing the quantification of Fis1 expression in the ganglion cell layer. There is a significant decrease in Fis1 expression in the ganglion cell layer of the old retina when compared to the young (P = 0.0048). C. Graph showing that Fis1 is significantly increased in the inner segments of the photoreceptor layer (P = 0.0333). D. Western blot results for the Fis1 protein showed that Fis1 in the old retina is reduced by 60% when compared to young retinae (P = 0.0910). Mean ± SEM. Scale bar = 25μm. GCL; ganglion cell layer, IPL; Inner plexiform layer, INL; inner nuclear layer, OPL; outer plexiform layer, ONL; outer nuclear layer, PR; photoreceptor layer and RPE; retinal pigment epithelial.