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. 2015 Sep 10;6(29):26690–26701. doi: 10.18632/oncotarget.5614

Figure 4. Changes in levels of mitochondrial stress marker Hsp60 in the ageing retina of C57BL/6 mice.

Figure 4

A. Retinal sections of young and old mice immunostained for Hsp60 (red) and the nuclei counterstained with 4′, 6-diamino-2-phenylindole (DAPI) (Blue). Hsp60 is strongly expressed in the inner plexiform layer and outer plexiform layer of the young mice compared to old. B. Quantification of the Hsp60 expression in the inner plexiform layer showed that it was significantly reduced in old compared with young (P = 0.0043). C. Quantification of Hsp60 in the outer plexiform layer showed a significant reduction in the old retina compared to the young (P = 0.0022) D. Western blot analysis showed a reduction in retinae of old mice which was not statistically significant (P = 0.0928). ). Mean ± SEM. Scale bar = 25μm. GCL; ganglion cell layer, IPL; Inner plexiform layer, INL; inner nuclear layer, OPL; outer plexiform layer, ONL; outer nuclear layer, PR; photoreceptor layer and RPE; retinal pigment epithelial.