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. 2015 Aug 22;6(29):26909–26921. doi: 10.18632/oncotarget.4817

Figure 2. Generation of A549 cell lines stably expressing shRNA constructs targeting genes of the MVA pathway.

Figure 2

A-B. RNA and protein lysates were harvested from asynchronously growing A549 cells that express two independent control shRNAs (shControl A, shControl B) or shRNAs targeting SREBP2, GGPS1 or HMGCS1 (shRNA A, shRNA B). Real-time mRNA EXPRESSION data represents the mean ± SD of three independent experiments. Immunoblots are representative of at least three independent experiments. C. Knockdown of SREBP2 and GGPS1 significantly decreased fluvastatin IC50 values. *p < 0.05 (one-way ANOVA with Tukey post-test). Representative dose-response curves assessed using the MTT assay are shown in C. (right panel).