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. 2015 Aug 22;6(29):26909–26921. doi: 10.18632/oncotarget.4817

Figure 3. shRNAs targeting MVA pathway gene hits potentiate fluvastatin-induced apoptosis.

Figure 3

A. Exposure to fluvastatin (10 μM) for 72 hours of A549 cell lines stably expressing the indicated shRNA significantly induced apoptosis. B. Fluvastatin-induced apoptosis 72 hours post-treatment in the shGGPS1 cell lines was reversed by the concomitant addition of GGPP (10 μM) and not MVA (100 μM) but by both GGPP and MVA in shSREBP2 and shHMGCS1 cell lines. Bars represent the mean ± SD of at least three independent experiments * # p < 0.05 (*one-way ANOVA with Tukey-post test within each shA549 cell line comparing all groups, the fluvastatin-treated group being significantly different from the others, # the fluvastatin-treated group being significantly different than the ethanol and fluvastatin + GGPP groups). C. Protein lysates harvested from A549 cell lines treated with 10 μM of fluvastatin for 72 hours were immunoblotted and probed for PARP expression. Immunoblots are representative of at least three independent experiments.