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. 2015 Aug 22;6(29):26909–26921. doi: 10.18632/oncotarget.4817

Figure 6. Knockdown of SREBP2 abrogates the sterol-feedback loop and impairs 3D growth upon fluvastatin treatment.

Figure 6

Cells were treated for 24 hours with 10 μM of fluvastatin or ethanol control and assessed for expression of A. HMGCR B. HMGCS1 relative to GAPDH in shA549 cell lines. Bars represent the mean ± SD of at least three independent experiments *p < 0.05 (t-test, unpaired, two-tailed, comparison made within each cell line). C. Protein lysates harvested from A549 cell lines treated with 10 μM of fluvastatin (Fluva.) for 24 hours were immunoblotted for SREBP2 expression. Immunoblots are representative of at least three independent experiments. D. Treatment with fluvastatin for 72 hours with the indicated fluvastatin concentrations disrupts A549 acini in shSREBP2 cell lines in 3D culture. Cells were imaged using an AxioObserver and are representative of three independent experiments. Scale bar represents 200 μm in main image and 100 μm in inset.