Skip to main content
. 2015 Aug 22;6(29):26909–26921. doi: 10.18632/oncotarget.4817

Figure 7. Knockdown of SREBP2 abrogates the sterol-feedback loop and impairs 3D growth of MCF7 cells upon fluvastatin treatment.

Figure 7

A. Cells were treated for 24 hours with 10 μM of fluvastatin or ethanol control and assessed for expression of HMGCR (A), HMGCS1 (B) relative to GAPDH in shMCF7 cell lines. Bars represent the mean ± SD of at least three independent experiments *p < 0.05 (t-test, unpaired, two-tailed, comparison made within each cell line). B. Treatment with fluvastatin for 72 hours with the indicated concentrations disrupts MCF7 acini in the shSREBP2 cell line in 3D culture but not in the shControl cell line. Images are representative of three independent experiments composed of three technical replicates. Scale bar represents 200 μm in main image and 100 μm in inset.