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. 2015 Aug 10;6(29):26971–26981. doi: 10.18632/oncotarget.4699

Figure 4. Effects of miR-21 depletion in U251 glioma cells.

Figure 4

A. Western blotting of miR-21 target genes of RECK, PDCD4 and PTEN following miR-21 inhibition at 48 h. B. Cellular and exosomal mRNA levels of PTEN, RECK and PDCD4 as determined by RT-qPCR (*p < 0.05, **P < 0.01). C. Flow cytometry of Annexin V staining for the evaluation of cell apoptosis following miR-21 inhibition.