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. 2015 Aug 12;6(29):27023–27036. doi: 10.18632/oncotarget.4809

Figure 1. EZH2 and H3K27me3 are overexpressed in both mutant and WT melanoma cell lines but Y646 mutants are more sensitive to EZH2 inhibition.

Figure 1

Western blot of endogenous EZH2 and the downstream H3K27me3 mark in a panel of melanoma cell lines. EZH2Y646 mutants are highlighted in red, human epithelial melanocytes (HEM) and human dermal fibroblasts (HDF) were used as untransformed, normal cells. β-actin was used as a loading control for total cell lysates whereas total histone 3 (H3) was used for purified histones A. Dose dependent reduction of H3K27me3 in IGR1Y646 mutant cells treated with GSK126 for 48 hr compared to vehicle treated control (DMSO) B. IC50 values of GSK126 following 72 hr of treatment using a cell titer glow (CTG) viability assay C.