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. 2015 Aug 4;6(29):27113–27129. doi: 10.18632/oncotarget.4729

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Copyright: © 2015 Lu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. BEAS-2B cells were stably transfected with pcDNA3.1 vector/pcDNA3.1-SIRT1 or pGPU6 vector/shRNA-SIRT1. The success of transfection was confirmed by Western blotting. The whole-cell extracts were probed with anti-SIRT1 antibody. B. Cell migration behaviors in the stable pcDNA3.1 vector/pcDNA3.1-SIRT1 transfected BEAS-2B cells were evaluated by wound-healing assay. The pictures were taken at different time. C. Wound healing assay of pGPU6 vector/shRNA-SIRT1 transfected cells. Quantification(closure rate%) were obtained from three independent experiments and expressed as mean ± S.D. (n = 3). *p < 0.05 and **p < 0.01. D–E. Cell migration and invasion ability were analyzed by transwell assay. Results were expressed as mean ± S.D. (n = 3). The numbers of cells were obtained from three independent experiments. **p < 0.01.

A. BEAS-2B cells were stably transfected with pcDNA3.1 vector/pcDNA3.1-SIRT1 or pGPU6 vector/shRNA-SIRT1. The success of transfection was confirmed by Western blotting. The whole-cell extracts were probed with anti-SIRT1 antibody. B. Cell migration behaviors in the stable pcDNA3.1 vector/pcDNA3.1-SIRT1 transfected BEAS-2B cells were evaluated by wound-healing assay. The pictures were taken at different time. C. Wound healing assay of pGPU6 vector/shRNA-SIRT1 transfected cells. Quantification(closure rate%) were obtained from three independent experiments and expressed as mean ± S.D. (n = 3). *p < 0.05 and **p < 0.01. D–E. Cell migration and invasion ability were analyzed by transwell assay. Results were expressed as mean ± S.D. (n = 3). The numbers of cells were obtained from three independent experiments. **p < 0.01.