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. 2015 Aug 4;6(29):27113–27129. doi: 10.18632/oncotarget.4729

Figure 5. TNF-α played a role in SIRT1 promoting BEAS-2B cell migration and invasion upon B[a]P exposure.

Figure 5

A. The levels of TNF-α mRNA were detected by RT-PCR in the SIRT1 (PLKO.1 vector)/SIRT1 (TNF-α silencing) or SIRT1 silencing (pcDNA3.1 vector)/SIRT1 silencing (TNF-α) transfected cells. B. and C. Cell migration behaviors of the above cells were tested by wound-healing assay. D. Cell invasion ability of the above cells were tested by transwell assay. E. Comparing the cell migration behaviors of TNF-α silencing (pCDNA3.1/pcDNA3.1-SIRT1 and pGPU6/shRNA-SIRT1) cells with or without B[a]P treatment by wound-healing assay. Results were expressed as mean ± S.D. (n = 3). The numbers of cells were obtained from three independent experiments.