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. 2015 Aug 4;6(29):27113–27129. doi: 10.18632/oncotarget.4729

Figure 6. SIRT1 promoted β-catenin accumulation upon B[a]P exposure.

Figure 6

A. The expression of the invasion-associated genes (Ras-related C3 botulinum toxin substrate 1, RAC1/Cell division cycle 42, CDC42/Cofilin2, CFL2/β-catenin in the pGPU6 vector/pGPU6-SIRT1 transfected cells under B[a]P exposure were examined by RT-PCR. B. The expression levels of β-catenin mRNA in the pcDNA3.1 vector/pcDNA3.1-SIRT1 transfected cells were detected by RT-PCR. C. The stable transfection cells of pcDNA3.1-SIRT1 and its vector were exposed to B[a]P as indicated. The protein levels of β-catenin were determined by Western blotting. D. The nuclear and plasma levels of β-catenin in SIRT1 overexpression, SIRT1-shRNA and their comparing vectors were determined by Western blotting.