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. 2015 Aug 11;6(29):27130–27145. doi: 10.18632/oncotarget.4825

Figure 6. The synergistic regulation of hTERT promoter activity and hTERT expression in H460 cells by CBP and RFPL3.

Figure 6

A. H460 cells were co-transfected with RFPL3 plasmid and CBP-specific siRNA1, or CBP inhibitor. The level of RFPL3 in the proteins pulled down by hTERT promoter probes was determined by immunoblot. B. H460 cells co-transfected with RFPL3 plasmids and CBP-specific siRNA1, or CBP inhibitor were co-incubated with the plasmids of hTERT promoter driven-luciferase for 48 hours. The luciferace activity was measured. C. H460 cells were co-transfected with CBP plasmids and RFPL3-specific siRNA and the plasmids of hTERT promoter driven-luciferase for 48 hours followed by luciferace activity assays. D. H460 cells were co-transfected with RFPL3 plasmid and CBP-specific siRNA1, or CBP inhibitor. The expressions of hTERT, RFPL3 and CBP proteins were examined by western blot. E. H460 cells were co-transfected with CBP plasmids and RFPL3-specific siRNAs. The expressions of hTERT, RFPL3 and CBP proteins were examined by western blot.