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. 2015 Sep 3;6(29):27187–27198. doi: 10.18632/oncotarget.4551

Figure 3. Tumor suppressive functions of si-EpCAM in breast cancer cells.

Figure 3

A. MCF-7 and MDA-MB-231 cells were treated with control siRNA or siRNA against EpCAM (0 ng, 1.0 ng, 2.0 ng respectively). qRT-PCR (only in MCF-7 cells) and Western blot analysis were performed to detect EpCAM expression. GAPDH was used as a loading control. *P < 0.01. B. Cell growth rates were determined with a CCK-8 proliferation assay. *P < 0.01. C. Representative increase of foci formation in monolayer culture by si-EpCAM and quantitative analyses of foci numbers. Columns, mean of at least three independent experiments; bars, standard deviation. ***P < 0.001. D. Effects of si-EpCAM on Ki67 in breast cancer cell lines. Ki67 expression was detected by immunofluorescence staining in MCF-7 and MDA-MB-231 cells treated with si-EpCAM transfection. Red fluorescence: Ki67; DAPI staining for nuclear DNA.