Figure 5. MiR-183 enhances the stability of HIF1α through targeting VHL mRNA CDS.

A. The schematic diagram shows the heteroduplexes of VHL mRNA CDS and its mutant in the miR-183 recognition elements (MREs). B, C. The relative luciferase activities of wild-type and mutant pLuc-VHL-397p-183 were detected by luciferase reporter gene assays in MCF-7 cells transfected with miR-183 (or in MCF-7-HBXIP cells transfected with anti-miR-183). D, E. The expression levels of pVHL were detected by Western blot analysis in MCF-7 cells transfected with miR-183 (or in MCF-7-HBXIP cells transfected with anti-miR-183). F, G. The expression levels of HIF1α were measured by Western blot analysis in MCF-7 cells transfected with miR-183 (or in MCF-7-HBXIP cells transfected with anti-miR-183). Statistically significant differences are indicated: **P < 0.01, Student's t-test. Each experiment was repeated at least three times. Data are shown as mean ± SEM (n = 3).