Skip to main content
. 2015 Aug 6;6(29):27214–27226. doi: 10.18632/oncotarget.4877

Figure 2. Resveratrol and pterostilbene rescue PTEN inhibition by oncomiRs -17, -20a and -106b.

Figure 2

A. Schematic representation of the predicted target sites of miRs-17, -20a and -106b in the 3′UTR of PTEN mRNA. The miRNA seed sequence is shared by all three miRs and shown in red. Mutated nucleotides in the 3′UTR are shown in bold. B, C. Resveratrol and pterostilbene oppose PTEN 3′UTR targeting. Relative luciferase activity in DU145 cells co-transfected with wt PTEN 3′UTR along with either pre-miR-17, -20a or 106b and treated with resveratrol (B) or pterostrilbene (C) D. Co-transfections with mutated 3′UTR did not show any inhibitory effect on luciferase activity. MiR-negative#1 was used as a negative control (miR-NC). Values are normalized to Renilla luciferase activity and relative to 3′UTR/EV (Empty Vector) ratio which is set at 1. Data represent the mean ± SEM from four independent experiments. Comparisons between non-transfected and miR transfected samples (#) and vehicle and compound-treated samples (*) are shown. #p < 0.05; ##p < 0.01; *p < 0.05; **p < 0.01. Res, resveratrol; Pter, pterostilbene.