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. 2015 Aug 7;6(29):27288–27303. doi: 10.18632/oncotarget.4803

Figure 1. Tumor sphere cultured (TSC) cells exhibit a cancer stem-like cell phenotype and chemoresistance.

Figure 1

A. Representative images obtained from primary lung adenocarcinoma murine Lacun.3 cells and human lung adenocarcinoma cell line H460 cultured under TSC conditions. B. RT-qPCR showed greater expression of the stem cell markers ALDH, ScaI in Lacun.3 and ALDH, OCT4 and ESA in H460 TSC versus adherent cultured (AC) cells. C. Analysis by flow cytometry showed higher protein expression levels of ScaI, ALDH and ABCG2, ALDH in Lacun.3 and H460 TSC respectively, as compared to AC cells. D. Left panel: Quantification of cell viability of TSC and AC cells after one week treatment with salinomycin (Sal) and paclitaxel (Pac) (***, p < 0.001). Right panel: Representative phase-contrast microscopy images of untreated (UT) and treated TSC with Sal and Pac. This experiment was repeated three times with similar results. E. Soft agar assay showing an increased anchorage-independent growth ability of TSC versus their matched AC cells. This experiment was repeated three times with similar results. (*p, 0.05; **p < 0.01; ***, p < 0.001), Error bars are mean ± SEM.