Figure 2. Cisplatin coordinately displaces UBF from the rRNA genes and arrests their transcription.

A. Timeline of cisplatin treatment and culture of Ubf^wt/wt/Er-cre^+/+/SvT iMEFs. B. ChIP analyses of UBF occupancy across the rRNA gene 47S transcribed region. The positions of amplicons is indicated above the histogram showing the UBF occupancy normalized to that in the mock treated cells. C. Synthesis rate of rRNA determined by [³H]-uridine metabolic labelling of mock treated cells and at the indicated times post cisplatin treatment. The upper panel displays a fluorogram of [³H]-rRNA, the central panel the corresponding EtBr stained total 18S rRNA, and the lower panel quantitation of [³H] incorporation into 47S rRNA performed in triplicate. D. Live cell counts at indicated times following cisplatin treatment performed in triplicate.