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. 2015 Jul 30;6(29):27651–27660. doi: 10.18632/oncotarget.4753

Figure 2. IL-6 protected A549 and H157 cells from apoptotic death after cisplatin treatment via up regulation of Bcl-2 and Mcl-1.

Figure 2

A. Analysis of apoptosis following cisplatin treatment. A549IL-6si/sc and H157IL-6si/sc cells were treated with cisplatin (5 μM) for 48 hours and subjected into Annexin V based-flow cytometric analysis according to the manufacturer's instruction. The % of apoptotic cell is indicated in each graph. B. qPCR results of analyzing mRNA expressions of anti-apoptotic molecules in A549IL-6si/sc and H157IL-6si/sc cells after cisplatin treatment (5 μM, 48 hours). C. Western blot results showing expression of anti-apoptotic proteins in A549IL-6si/sc and H157IL-6si/sc cells following cisplatin treatment (5 μM, 48 hours). D. IHC staining of tumor tissues of A549IL-6si/sc xenografts showing expression of Bcl-2 and Mcl-1(Magnification, 100x). Quantitation of Bcl-2 and Mcl-1 IHC staining is shown on right. *p < 0.05, **p < 0.01, ***p < 0.001.