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. 2015 Aug 7;6(29):27751–27762. doi: 10.18632/oncotarget.4859

Figure 1. DCs maturation, activation and common TLR signal pathway activation by PAUF protein.

Figure 1

A. DCs activation was demonstrated by measuring pro-inflammatory cytokines and type I interferon, DCs activation factors, in culture supernatant of DCs incubated with PAUF (1 μg or 10 μg) or LPS (100 ng) as a positive control for 16 hr with ELISA. B. and C. To probe maturation level of DCs, after DCs were incubated with PAUF or LPS for 16 hr, maturation surface markers (CD40, CD80, CD86, MHC class I) were confirmed by using flow cytometry analysis after incubation with PAUF or LPS as a positive control and PBS as a negative control. D. To determine MAPKs and IkB-α, intracellular protein marker of activated DCs, DCs were treated with PAUF (5 μg) of time course and analyzed by using Western Blot analysis. **: P < 0.01.