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. 2015 Aug 7;6(29):27751–27762. doi: 10.18632/oncotarget.4859

Figure 2. Human Dendritic cells can be activated and matured using PAUF protein.

Figure 2

A. To confirm maturation factor (CD80, CD83 and CD86) and migration factor (CCR7) of mature DCs compared with immature DCs (imDC), DCs (PAUF-DC1, PAUF-DC2, and PAUF-DC3) were treated with PAUF of various concentrations (5 μg, 10 μg and 20 μg/ml, respectively) or LPS (100 ng/ml; LPS-DC) for 2 days, and then analyzed by flow cytometry. Histogram shows antibody staining (in dark) relative to isotype-matched control (transparent). Data are mean fluorescence intensities (MFI) of three independent experiments. B. The bar graph depicts mean fluorescence intensity (MFI) of each surface markers. C. Cytokines (IL-12p70, IL-10 and IL-23) in the culture supernatant of PAUF (5 μg, 10 μg and 20 μg/ml) of various concentration or LPS (100 ng/ml)-treated DCs for 2 days were determined by ELISA. Immature DC culture supernatant was used as a control. **: P < 0.01.