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. 2015 Aug 7;6(29):27751–27762. doi: 10.18632/oncotarget.4859

Figure 3. PAUF mediated DC vaccine can generate antigen specific CD8+ T cell and memory CD8+ T cell immune response and has tumor prevention effects.

Figure 3

A. and C. E7 or OVA specific CD8+ T cells were counted in splenocytes of immunized mice after various conditional DC vaccines by using flow cytometry. B. and D. Kaplan-Meier survival analysis of mice. For in vivo tumor prevention effects against E7 expressing TC-1 cells and OVA expressing EG.7 cells, mice were immunized with DCs and then TC-1(2.5 × 105) or EG.7(5 × 106) tumor cells were subcutaneously challenged after 7 days last DCs vaccination. E. To demonstrate in vivo long-term antigen (E7 and OVA) specific memory CD8+ T cells generation after PAUF mediated DC vaccine, after seven weeks of last vaccination, activated antigen specific CD8+ T cells were counted in splenocytes of only immunized mice and one week after tumor challenged mice. Naïve mice and tumor challenged mice without vaccination were used as a control. F. The bar graph indicated the number of antigen specific CD8+ T cells in splenocytes. G. Kaplan-Meier survival analysis of mice. For the test of long-term tumor protection ability after PAUF mediated DC vaccination, mice were subcutaneously challenged with TC-1(1 × 105 cells/mouse) or EG.7(1 × 106 cells/mouse) seven weeks after last vaccination. Same numbers of tumor cells were injected into naïve mice as a control. **: P < 0.01.