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. 2015 Aug 7;6(29):27751–27762. doi: 10.18632/oncotarget.4859

Figure 5. PAUF mediated DC activation and maturation depend on TLR4.

Figure 5

A. To reconfirm binding of PAUF and TLR2 or TLR4 described previously, coherence between PAUF and TLR2 or TLR4 was determined with BLITZ. B. DCs maturation in wild type, TLR2−/− or TLR4−/− DCs after treatment with PAUF or LPS as described in Figure 1. C. The bar graph depicts mean fluorescence intensity of each surface marker. D. Bar graph depict amount of pro-inflammatory cytokines and type I interferon in wild type, TLR2−/− and TLR4−/− DCs after treatment with PAUF or LPS as described in Figure. E. MAPKs and IkB-α of TLR signal pathway, confirmation marker of activated-DCs, were determined by using Western Blot analysis as described in the materials and methods. **: P < 0.01.