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. 2015 Jun 30;6(29):27847–27864. doi: 10.18632/oncotarget.4443

Figure 6. The HOTAIR inhibits the hMSH2/6-H3k36me3-Skp2 ternary complex loading onto the injury DNA in human liver cancer stem cell (hLCSC).

Figure 6

A. The schematic illustrates a model that injury plasmids (EcoRI mismatch) was repaired after the plasmid was transfected and intergrated into chromosome. B. Chromatin Immunoprecipitation (CHIP) with anti-.SKP2, anti-hMSH2, anti-hMSH6, anti-H3K36me3 followed by PCR with damaged DNA primers. IgG CHIP as negative control. damaged DNA as INPUT. C. Chromatin Immunoprecipitation (CHIP) with anti-SKP2, anti-hMSH6, anti-hMSH6, anti-H3K36me3 followed by PCR with match double DNA primers. IgG CHIP as negative control. match double DNA DNA as INPUT. D. Restriction endonuclease analysis with BamHI and EcoRI for DNA injury repair. E. Restriction endonuclease analysis with BamHI and EcoRI for DNA injury imrepair.