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. 2015 Jul 20;6(29):28011–28025. doi: 10.18632/oncotarget.4377

Figure 4. Effect of TUDCA on DEN-induced hepatic inflammation.

Figure 4

A. Immunostaining for F4/80 and B. quantification of F4/80-positive macrophages in the liver after the indicated treatments (n = 5). C. Determination of the indicated hepatic cytokine levels by multiplex microbead immunoassay (n = 8). Values represent the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. D. Expression of IκBα, phospho-NFκB p65 and NFκB p65 was detected using Western blotting. Results are representative of 2 independent experiments. E. Phospho-NFκB p65/ NFκB p65 ratio obtained using densitometric analysis of the Western blot shown in D.