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. 2015 Aug 27;6(29):28120–28131. doi: 10.18632/oncotarget.4866

Figure 1. Melanocytes are more sensitive to dual PI3K/mTOR inhibition than PI3K inhibition due to a feedback mechanism inducing AKT reactivation.

Figure 1

A. NHEMs were treated for 3 days with different concentrations of LY294002, OSI-027 or GDC-0980 in growing medium and proliferation was analyzed with CellTiter (data are represented as mean +/− SD). B. NHEMs were treated for 5, 15, 30, 90 minutes with DMSO (control), 5 μM LY294002 or 0.5 μM GDC-0980. Levels of phosphorylated proteins and total proteins were analyzed by Western Blotting (data are represented as mean +/− SD). C. Melan-a cells were treated for 15 min or 1 h30 with LY294002 or GDC-0980 and interaction between RICTOR and AKT was studied by proximity ligation assay. D. NHEMs were treated for 5, 15, 30, 90 minutes with DMSO (control), 5 μM LY294002 or 0.5 μM GDC-0980. Levels of phosphorylated protein or total protein were analyzed by Western Blotting.