Figure 1. MVA-TWIST/TRICOM expresses Twist and a TRiad of COstimulatory Molecules (TRICOM).
Western blot and flow cytometric analysis of MC38 cells treated with A. no treatment, B. MVA-TRICOM or C. MVA-TWIST/TRICOM for 24 hours. Antibodies against Twist and GAPDH were used for western blots. Antibodies against murine B7-1, ICAM-1, LFA-3 and isotype controls were used for flow cytometry. Inserts: percent of cells positive for the indicated marker and its mean fluorescent intensity (MFI). Data are representative of two independent experiments.