Figure 3. Treatment with MVA-TWIST/TRICOM induces Twist-specific T-cell responses in 4T1 tumor-bearing BALB/c mice.

BALB/c mice (n = 5/group) were implanted s.c. with 5 × 10⁴ 4T1 cells. Four days post-tumor implantation, mice received the first of two weekly MVA-TWIST/TRICOM (black bars) or were left untreated (open bars). A. The level of CD4⁺ T-cell proliferation was determined by [³H]-thymidine incorporation in the presence of irradiated antigen-presenting cells and Twist peptide. B. The level of CD8⁺ T-cell activity was determined by the amount of secreted IFN-γ following 7 days of in vitro Twist peptide stimulation. C. The level of CD8⁺ T-cell activity was determined by the amount of secreted IFN-γ following 7 days of in vitro AH-1 peptide stimulation. Error bars indicate mean ± S.E.M. from triplicate measurements. Statistical analyses for CD4⁺ T-cell proliferation were done by Student’s t-test, * = P < 0.05. For IFN-γ secretion, significance was determined by the Kolmogorov-Smirnov test.