Table 1. Splenic immune cell subset changes in non-tumor bearing BALB/c mice after administration of MVA-TWIST/TRICOM.

	CD4+ T cells	CD8+ T cells	Effector Memory CD8+ T cells	Twist Tetramer+ CD8+ T cells	Dendritic cells	Activated Dendritic cells
No Treatment	26.10 (1.017)	10.92 (0.2853)	0.7470 (0.0473)	2.43	1.390 (0.1243)	0.2592 (0.0762)
MVA-TWIST/TRICOM	26.78 (0.5093)	12.22 (0.2131)	1.56 (0.1597)*	3.83*	1.410 (0.07629)	0.5564 (0.0702)*

Flow cytometric analysis of splenic immune cell subsets in BALB/c mice (n = 5/group). Animals were administered two weekly vaccinations with MVA-TWIST/TRICOM or left untreated. Spleens were harvested 17 days after the last vaccination. Data represented as “mean (SEM)” percentage of viable splenocytes. CD4+ T cells (CD3+ CD4+), CD8+ T cells (CD3+ CD8+), Effector Memory cells (CD3+CD8+CD44+CD62L-), Dendritic cells (CD11c+MHCII+) and Activated Dendritic cells (CD11c+MHCII+CD40+).

^*= p < 0.01 as determined by Student’s t test or the Kolmogorov-Smirnov test.