Table 2. Splenic immune cell subset changes in 4T1-tumor bearing BALB/c mice after administration of MVA-TWIST/TRICOM.

	CD4+ T cells	CD8+ T cells	Effector Memory CD8+ T cells	Central Memory CD8+ T cells	Dendritic cells	Activated Dendritic cells
No Treatment	7.854 (1.396)	3.212 (0.4886)	0.5732 (0.116)	1.182 (0.1463)	1.090 (0.1974)	0.3352 (0.0745)
MVA-TWIST/TRICOM	14.10 (1.684)*	6.224 (0.9233)*	1.411 (0.2882)*	2.484 (0.4521)*	1.166 (0.1646)	0.3026 (0.0736)

Flow cytometric analysis of splenic immune cell subsets in 4T1-tumor bearing BALB/c mice (n = 5/group). Four days post-tumor implantation, mice were administered two weekly vaccinations with MVA-TWIST/TRICOM or left untreated. Spleens were harvested 17 days after the last vaccination. Data represented as “mean (SEM)” percentage of viable splenocytes. CD4+ T cells (CD3+ CD4+), CD8+ T cells (CD3+ CD8+), Effector Memory cells (CD3+CD8+CD44+CD62L-), Central Memory cells (CD3+CD8+CD44+CD62L+), Dendritic cells (CD11c+MHCII+) and Activated Dendritic cells (CD11c+MHCII+CD40+).

^*= p < 0.01 as determined by Student’s t test.