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. 2015 Jul 22;6(29):28223–28237. doi: 10.18632/oncotarget.4950

Figure 2. ZNF224 expression in drug-treated K562 cells.

Figure 2

K562 cells were exposed to increasing concentration of imatinib (0.3 μM, 0.6 μM, 1 μM, 3 μM) or vehicle only (DMSO) as control and analyzed after 24, 48 and 72h. a. Apoptosis was determined by annexin V-APC staining followed by flow cytometry. Results represent the mean +/− SD of three independent experiments. b. ZNF224 mRNA levels were measured by RT-qPCR. Relative amounts as compared to control are shown. Error bars represent standard deviations of three independent experiments. c. d. K562 cells were treated with 20 μM Etoposide (ETO), 10 μg/ml Camptothecin (CPT), 5 μM Staurosporine (STS) or vehicle only (DMSO) as control and analyzed for apoptosis as determined by Annexin V-APC staining followed by flow cytometry (c) and ZNF224 mRNA levels as determined by RT-qPCR analysis (d) after 24 and 48h. Results represent the mean +/− SD of two independent experiments.