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. 2015 Jul 31;6(29):28296–28311. doi: 10.18632/oncotarget.5064

Figure 7. Effects of fisetin, sorafenib and their combination on MAPK and PI3K signaling pathways, and angiogenesis in tumor tissues of athymic nude mice implanted with BRAF mutated melanoma cells.

Figure 7

Tumors from athymic nude mice implanted with melanoma cells and treated with the vehicle or fisetin, sorafenib and their combination were harvested for immunostaining and Western blot analyses. A. & B. Protein lysates were examined for molecules of the MAPK and PI3K signaling pathways. Equal protein loading was confirmed by stripping the immunoblot and reprobing it for β-actin. The data shown here are from a representative experiment repeated three times with similar results. C. & D. Sections from tumors harvested form mice treated with vehicle or fisetin, sorafenib and their combination were stained for angiogenesis markers (CD31 and VEGF) as described earlier. Bar = 20μm.