Table 3. Correlation between RK-33 sensitivity and mutation status in colorectal cancer cell lines.
| Cell line | RK-33 Sensitivity | Mutations | MSI status | ||||
|---|---|---|---|---|---|---|---|
| IC50 (μM) | 95% CI | APC Truncation | TP53 | RAS/RAF | Others | ||
| HCT116* | 2.71 | 2.64–2.80 | − | − | KRAS | PIK3CA, CTNNB1 | MSI |
| CRC29† | 2.89 | 2.66–3.14 | − | − | KRAS, BRAF | SMAD4, STK11 | MSI |
| HT29* | 2.96 | 2.84–3.08 | E853X, T1556fsX3 | R273H | BRAF | PIK3CA, SMAD4 | MSS |
| CR9† | 4.97 | 4.51–5.48 | R1114X, S1503E | R213X, V157A | − | SMAD4, FBXW7, ERBB2 | |
| DLD-1* | 5.36 | 5.15–5.59 | I1417X | S241F | KRAS | MSI | |
| CRC47† | 5.54 | 5.07–6.05 | R1450X | P75LfsX48 | − | PIK3CA | MSI |
| SW480* | 6.32 | 5.60–7.15 | Q1338X | R273H, P309S | KRAS | SMAD4 | MSS |
| Colo205* | 6.65 | 6.00–7.37 | T1556fsX3 | Y103fsX8 | BRAF | MSS | |
| L145† | 8.77 | 7.63–10.08 | S1436LfsX34 | R110P | KRAS | MSS | |
The genomic background of colorectal cancer derived adherent cell lines and 3D cultures and their relative sensitivity to RK-33. Mutational status was derived from:
*
publicly available data in the CanSAR database.
†
next-generation sequencing.
−no mutation detected.