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. 2015 Jul 22;6(29):28371–28388. doi: 10.18632/oncotarget.4948

Figure 5. VTRNA2-1-5p promotes tumor cell growth in vitro and in vivo.

Figure 5

A. Colony formation assays were performed on SiHa and HeLa cell lines untreated or transfected with the negative control or the VTRNA2-1-5p mimic or inhibitor (n = 6). B. SiHa cells in 6-well plates were stably transfected with 1.0 μg VTRNA2-1-5p inhibitor GFP plasmid or negative control GFP tag vector, and inhibition was evaluated by real-time stem-loop PCR (n = 3). C. Stably transfection cells (n = 6) were examined for cell viability using the MTT assay as described in the Materials and Methods section. D. Xenografts in nude mice. Untreated, negative control vector transfected, and VTRNA2-1-5p-inhibitor transfected SiHa cells were s.c. injected into the posterior flanks of nude mice (n = 5). The graph shows data obtained from tumor tissues extracted from the various groups after 2 weeks. The tumor volumes were calculated, and the data are presented as the mean±SD. H&E staining was performed on mouse tumor tissue untreated or transfected with the VTRNA2-1-5p inhibitor or negative control vector. Magnification×200 (H&E). The two-tailed Student’s t-test was used.