Skip to main content
. 2015 Jul 22;6(29):28401–28424. doi: 10.18632/oncotarget.4951

Figure 3. Stromal S100A9 expression in monocytes promotes the migration and invasion of co-cultured oral cancer cells.

Figure 3

Figure 3

Figure 3

Figure 3

Figure 3

A. S100A9 protein was detected both in tumor and stroma of one represent oral cancer specimen by IHC staining (100X magnification). B. The presence of S100A9 protein and indicative myeloid cell markers in tumor stroma by confocal immunofluorescence staining of serial sections of the oral cancer specimen. CD15, neutrophils; CD11b, monocytes; CD68, macrophages; DAPI, nuclear stain. C. The increase of ectopic S100A9 protein in the indicated U937 cell clones detected by Western blot analysis. Actin serves as a loading control. D. TW2.6-mCherry cells were incubated with the indicated U937 cells in 2:1 ratio for 48 hours. The number of mCherry-positive cancer cells was measured by a SpectraMAX M3 microplate reader. Data are mean ± SD. E. Cell migration and invasion abilities of TW-2.6 cells co-cultured with indicated U937 cells were measured by using Transwell plates. TW-2.6 cells seeded in the inserts were incubated with the indicated U937 cells in the bottom wells for 24 hours. Data are mean ± SEM. *p < 0.05 or ***p < 0.001 versus vector.