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. 2015 Dec 29;10(12):e0141960. doi: 10.1371/journal.pone.0141960

Fig 3. Effects of RXR agonists on Pomc mRNA expression, ACTH secretion, and Pomc promoter activity in AtT20 cells.

Fig 3

In A and B, total RNAs extracted from the cells cultured for 48 hr either in the presence (100 nM, 1 μM, 5 μM, or 10 μM) or absence (control) of each RXR agonist, HX630 and PA024, respectively, were examined for Pomc mRNA expression by quantitative real-time PCR. In C and D, supernatants obtained from the cells cultured for 48 hr either in the presence (100 nM, 1 μM, 5 μM, or 10 μM) or absence (control) of each RXR agonist, HX630 and PA024, respectively, were examined for ACTH secretion by EIA. In E and F, AtT20 cells transiently transfected for 24 hr with rPomc-luc (-703 to +58-luc) and pCMV-β-gal were incubated either in the presence (100 nM, 1 μM, 5 μM, or 10 μM) or absence (control) of each RXR agonist, HX630 and PA024, respectively, before the luciferase assay. Results are expressed as percentages of each control. Each point represents mean ± SEM (n = 4). *P<0.05 vs control of each RXR agonist.