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. 2015 Jul 27;6(27):23238–23248. doi: 10.18632/oncotarget.4836

Figure 4. Torin 1 and PP242 suppress geroconversion in human WI38t fibroblasts and SkBr3 cells.

Figure 4

A. WI38t cells were treated with either 1 μg/ml etoposide or 50 ng/ml doxorubicin in the absence or presence of 100 nM Torin 1 or 1 μM PP242 for 4 days and stained for beta-Gal. Bar – 100 μm. B. Cells were treated as in (A). After 4-day treatment, cells were extensively washed to remove the drugs and allowed to regrow in drug-free medium and counted after 3 weeks in culture. RP (re-proliferative potential) was calculated by dividing final cell numbers by initially-plated number of cells. Data present mean ±SD from triplicate wells. C. Effect of Torin 1 on senescent morphology of SKBR3 cells undergoing senescence induced by PMA. Cells were pre-treated with Torin 1 (100 nM) for 24 h before addition of 100 nM PMA. After 3-day treatment with PMA, drugs were washed out, cells were incubated in drug-free medium for another 3 days and stained for beta-gal. Bar – 100 μm.