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. 2015 Jun 19;6(27):23548–23560. doi: 10.18632/oncotarget.4556

Figure 7. Hyperglycemia inhibits the cytotoxic effect of metformin in vivo.

Figure 7

A. Viability of ID8 cells treated in vitro with 5 mM metformin in normoglycemic (5.5 mM glucose) or hyperglycemic (25 mM glucose) conditions for 72 h. Values are mean ± SEM, n = 3. *p < 0.05 or **p < 0.0001 between the indicated values. B. Glucose tolerance test (0-120 min) performed on normoglycemic or hyperglycemic C57BL/6J mice following intraperitoneal injection of 2 g/kg D-glucose. Blood glucose levels were significantly different between normoglycemic and hyperglycemic mice at baseline (0 min) and 90 min post-injection of D-glucose (p < 0.01). C. Mean tumor weight in normoglycemic or hyperglycemic C57BL/6J mice injected orthotopically with ID8 ovarian cancer cells in the ovarian bursa and treated with intraperitoneal metformin (200 mg/kg) or placebo (PBS) daily. *p < 0.05 or **p < 0.01 between the indicated values. D. Western blot of pAMPK Thr172 (62 kDa), AMPK (62 kDa), c-Myc (57-65 kDa), and β-actin (42 kDa) in tumor lysates from normoglycemic or hyperglycemic mice treated with placebo or metformin (n = 2 from each group).