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. 2015 Jun 8;6(27):23662–23670. doi: 10.18632/oncotarget.4282

Figure 1. CD47 is highly expressed in osteosarcoma.

Figure 1

Quantitative RT-PCR A. and immunoblot analysis B. showed CD47 expression in ten freshly isolated osteosarcoma tissues and their adjacent nontumorous tissues. Beta-actin was treated as the reference control. CD47 immunostaining C. showed higher of CD47 expression in osteosarcoma of the same samples with or without metastasis and their adjacent nontumorous tissues. The tissues were counterstained with DAPI Fluorescent Stain. Representative images are shown here. (magnification × 100). D. Flow-cytometry analysis of CD47 and CD44 expressions on osteosarcoma tumor cells and quantification of CD44 cells also expressing CD47.