Skip to main content
. 2015 Jun 8;6(27):23662–23670. doi: 10.18632/oncotarget.4282

Figure 5. CD47 blockade using specific antibodies increases macrophage phagocytosis of osteosarcoma tumor cells.

Figure 5

A. LM8 tumor cells were labeled with CFSE and cultured with peritoneal-derived macrophages from NSG mice. The cells were incubated with control IgG or blocking B6H12 anti-CD47 antibody. Representative 200 × magnification images are shown for visualization by spinning disk confocal microscopy. Incubation with blocking anti-CD47 antibody resulted in increased phagocytosis of CFSE labeled LM8 cells (arrows). Bars, 25 μm. The extent of macrophage phagocytosis with each antibody was quantified for LM8 (B. p < 0.001) and KRIB cells (C. p < 0.001).