Skip to main content
. 2015 Jun 3;6(27):23776–23792. doi: 10.18632/oncotarget.4196

Figure 7. DIM inhibited cell migration and invasion through suppressing the activity of FAK pathway and MMP2/9 expression induced by vitronectin.

Figure 7

SMMC-7721 and MHCC-97H cells were treated with DIM (10, 15 and 20 μM) on VTN-coated or BSA-coated plate for 48 h. A. Western blotting analysis of cell lysates for FAK and FAP(Y397)-p, MMP-2 and MMP-9. B. Western blotting and zymography assay of MMP-2 and MMP-9. C. Cells invasion was performed via transwell inserts with basement membrane extract. SMMC-7721 and MHCC-97H (1 × 105 cells per well) were seeded in insert in 200 μl no-serum medium containing different concentration of DIM (10, 15 and 20 μM), the bottom wells were loaded with 800 μl no-serum medium containing 5 ng/ml vitronectin or 5 ng/ml BSA and cells were incubated for 24 h. Cells were stained with Giemsa. Values represent mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the untreated control (dose 0).