Table 2. Gene mutations result analyzed by Sanger sequencing.
| Gene | Analyzed mutation points | N of mutation | Codon | Subtotal in codon | Total | Overall mutation rate (%) |
|---|---|---|---|---|---|---|
| BRAF | c.1798G>A/T | 0 | V600 | 5 | 5 | 3.94 |
| c.1799T>A | 4 | |||||
| c.1799T>G | 1 | |||||
| c.1799T>C | 0 | |||||
| KRAS | c.34G>A/C/T | 10 | G12 | 38 | 52 | 40.94 |
| c.35G>A/C/T | 28 | |||||
| c. 37G>A/C/T | 1 | G13 | 10 | |||
| c. 38G>A/C/T | 9 | |||||
| c. 181C>A/G/T | 0 | Q61 | 2 | |||
| c. 182A>C/G/T | 0 | |||||
| c. 183A>C/T | 2 | |||||
| c.436G>A/C | 1 | A146 | 2 | |||
| c.437C>T | 1 | |||||
| NRAS | c. 34G>A/C/T | 1 | G12 | 2 | 3 | 2.36 |
| c. 35G>A/C/T | 1 | |||||
| c.37G/38G>A/C/T | 0 | G13 | 0 | |||
| c.181C>A/G/T | 0 | Q61 | 1 | |||
| c. 182A>C/G/T | 1 | |||||
| c.183A>C/T | 0 | |||||
| PIK3CA | c.1624G>A/C | 2 | E542 | 2 | 7 | 5.51 |
| c.1633G>A/C | 0 | E545 | 0 | |||
| c.1636C>A/G | 1 | E546 | 2 | |||
| c.1637A>C/G/T | 1 | |||||
| c.3139C>T | 0 | H1047 | 3 | |||
| c.3140A>G/T | 3 | |||||
| c.3145G>A/C | 0 | G1049 | 0 |
Abbreviations: BRAF, v-raf murine sarcoma viral oncogene homolog B; KRAS, Kirsten rat sarcoma viral oncogene homolog; NRAS, neuroblastoma RAS viral oncogene homolog; PIK3CA, phosphatidylinositol-4, 5-bisphosphate 3-kinase, catalytic subunit a.